看看有多少個鏈?
除了 protein/DNA 鏈chains之外 ，有沒有其它的東西?
呈現疏水性的胺基酸
哪裡是 a-螺旋和 b-長帶?
除了 protein/DNA 鏈chains之外 ，有沒有其它的東西?
呈現疏水性的胺基酸
哪裡是 a-螺旋和 b-長帶?
- Jun 29 Tue 2010 21:44
nr1h2 protein structure
- Jun 29 Tue 2010 21:19
nr1h2 protein MW, PI
以nr1h2 protein為例
將nr1h2 protein 序列以ExPASy分析其分子量及等電點
Compute pI/Mw
Theoretical pI/Mw (average) for the user-entered sequence:
MSSPTTSSLDTPLPGNGPPQPGAPSSSPTVKEEGPEPWPGGPDPDVPGTDEASSACSTDWVIPDPEEEPE
RKRKKGPAPKMLGHELCRVCGDKASGFHYNVLSCEGCKGFFRRSVVRGGARRYACRGGGTCQMDAFMRRK
CQQCRLRKCKEAGMREQCVLSEEQIRKKKIRKQQQQESQSQSQSPVGPQGSSSSASGPGASPGGSEAGSQ
GSGEGEGVQLTAAQELMIQQLVAAQLQCNKRSFSDQPKVTPWPLGADPQSRDARQQRFAHFTELAIISVQ
EIVDFAKQVPGFLQLGREDQIALLKASTIEIMLLETARRYNHETECITFLKDFTYSKDDFHRAGLQVEFI
NPIFEFSRAMRRLGLDDAEYALLIAINIFSADRPNVQEPGRVEALQQPYVEALLSYTRIKRPQDQLRFPR
MLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE
Theoretical pI/Mw: 7.90 / 51101.93
將nr1h2 protein 序列以ExPASy分析其分子量及等電點
Compute pI/Mw
Theoretical pI/Mw (average) for the user-entered sequence:
MSSPTTSSLDTPLPGNGPPQPGAPSSSPTVKEEGPEPWPGGPDPDVPGTDEASSACSTDWVIPDPEEEPE
RKRKKGPAPKMLGHELCRVCGDKASGFHYNVLSCEGCKGFFRRSVVRGGARRYACRGGGTCQMDAFMRRK
CQQCRLRKCKEAGMREQCVLSEEQIRKKKIRKQQQQESQSQSQSPVGPQGSSSSASGPGASPGGSEAGSQ
GSGEGEGVQLTAAQELMIQQLVAAQLQCNKRSFSDQPKVTPWPLGADPQSRDARQQRFAHFTELAIISVQ
EIVDFAKQVPGFLQLGREDQIALLKASTIEIMLLETARRYNHETECITFLKDFTYSKDDFHRAGLQVEFI
NPIFEFSRAMRRLGLDDAEYALLIAINIFSADRPNVQEPGRVEALQQPYVEALLSYTRIKRPQDQLRFPR
MLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE
Theoretical pI/Mw: 7.90 / 51101.93
- Jun 29 Tue 2010 21:15
以SMS找出nr1h2的ORF
ORF Finder results
Results for 1386 residue sequence "Untitled" starting "ATGTCCTCTC"
>ORF number 1 in reading frame 1 on the direct strand extends from base 1 to base 1386. ATGTCCTCTCCTACCACGAGTTCCCTGGATACCCCCCTGCCTGGAAATGGCCCCCCTCAG CCTGGCGCCCCTTCTTCTTCACCCACTGTAAAGGAGGAGGGTCCGGAGCCGTGGCCCGGG GGTCCGGACCCTGATGTCCCAGGCACTGATGAGGCCAGCTCAGCCTGCAGCACAGACTGG GTCATCCCAGATCCCGAAGAGGAACCAGAGCGCAAGCGAAAGAAGGGCCCAGCCCCGAAG ATGCTGGGCCACGAGCTTTGCCGTGTCTGTGGGGACAAGGCCTCCGGCTTCCACTACAAC GTGCTCAGCTGCGAAGGCTGCAAGGGCTTCTTCCGGCGCAGTGTGGTCCGTGGTGGGGCC AGGCGCTATGCCTGCCGGGGTGGCGGAACCTGCCAGATGGACGCTTTCATGCGGCGCAAG TGCCAGCAGTGCCGGCTGCGCAAGTGCAAGGAGGCAGGGATGAGGGAGCAGTGCGTCCTT TCTGAAGAACAGATCCGGAAGAAGAAGATTCGGAAACAACAGCAGCAGGAGTCACAGTCA CAGTCGCAGTCACCTGTGGGGCCGCAGGGCAGCAGCAGCTCAGCCTCTGGGCCTGGGGCT TCCCCTGGTGGATCTGAGGCAGGCAGCCAGGGCTCCGGGGAAGGCGAGGGTGTCCAGCTA ACAGCGGCTCAAGAACTAATGATCCAGCAGTTGGTGGCGGCCCAACTGCAGTGCAACAAA CGCTCCTTCTCCGACCAGCCCAAAGTCACGCCCTGGCCCCTGGGCGCAGACCCCCAGTCC CGAGATGCCCGCCAGCAACGCTTTGCCCACTTCACGGAGCTGGCCATCATCTCAGTCCAG GAGATCGTGGACTTCGCTAAGCAAGTGCCTGGTTTCCTGCAGCTGGGCCGGGAGGACCAG ATCGCCCTCCTGAAGGCATCCACTATCGAGATCATGCTGCTAGAGACAGCCAGGCGCTAC AACCACGAGACAGAGTGTATCACCTTCTTGAAGGACTTCACCTACAGCAAGGACGACTTC CACCGTGCAGGCCTGCAGGTGGAGTTCATCAACCCCATCTTCGAGTTCTCGCGGGCCATG CGGCGGCTGGGCCTGGACGACGCTGAGTACGCCCTGCTCATCGCCATCAACATCTTCTCG GCCGACCGGCCCAACGTGCAGGAGCCGGGCCGCGTGGAGGCGTTGCAGCAGCCCTACGTG GAGGCGCTGCTGTCCTACACGCGCATCAAGAGGCCGCAGGACCAGCTGCGCTTCCCGCGC ATGCTCATGAAGCTGGTGAGCCTGCGCACGCTGAGCTCTGTGCACTCGGAGCAGGTCTTC GCCTTGCGGCTCCAGGACAAGAAGCTGCCGCCTCTGCTGTCGGAGATCTGGGACGTCCAC GAGTGA
>Translation of ORF number 1 in reading frame 1 on the direct strand.
MSSPTTSSLDTPLPGNGPPQPGAPSSSPTVKEEGPEPWPGGPDPDVPGTDEASSACSTDW
VIPDPEEEPERKRKKGPAPKMLGHELCRVCGDKASGFHYNVLSCEGCKGFFRRSVVRGGA
RRYACRGGGTCQMDAFMRRKCQQCRLRKCKEAGMREQCVLSEEQIRKKKIRKQQQQESQS
QSQSPVGPQGSSSSASGPGASPGGSEAGSQGSGEGEGVQLTAAQELMIQQLVAAQLQCNK
RSFSDQPKVTPWPLGADPQSRDARQQRFAHFTELAIISVQEIVDFAKQVPGFLQLGREDQ
IALLKASTIEIMLLETARRYNHETECITFLKDFTYSKDDFHRAGLQVEFINPIFEFSRAM
RRLGLDDAEYALLIAINIFSADRPNVQEPGRVEALQQPYVEALLSYTRIKRPQDQLRFPR
MLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE*
>ORF number 1 in reading frame 2 on the direct strand extends from base 2 to base 91.
TGTCCTCTCCTACCACGAGTTCCCTGGATACCCCCCTGCCTGGAAATGGCCCCCCTCAGC
CTGGCGCCCCTTCTTCTTCACCCACTGTAA
>Translation of ORF number 1 in reading frame 2 on the direct strand.
CPLLPRVPWIPPCLEMAPLSLAPLLLHPL*
>ORF number 2 in reading frame 2 on the direct strand extends from base 92 to base 463.
AGGAGGAGGGTCCGGAGCCGTGGCCCGGGGGTCCGGACCCTGATGTCCCAGGCACTGATG
AGGCCAGCTCAGCCTGCAGCACAGACTGGGTCATCCCAGATCCCGAAGAGGAACCAGAGC
GCAAGCGAAAGAAGGGCCCAGCCCCGAAGATGCTGGGCCACGAGCTTTGCCGTGTCTGTG
GGGACAAGGCCTCCGGCTTCCACTACAACGTGCTCAGCTGCGAAGGCTGCAAGGGCTTCT
TCCGGCGCAGTGTGGTCCGTGGTGGGGCCAGGCGCTATGCCTGCCGGGGTGGCGGAACCT
GCCAGATGGACGCTTTCATGCGGCGCAAGTGCCAGCAGTGCCGGCTGCGCAAGTGCAAGG
AGGCAGGGATGA
>Translation of ORF number 2 in reading frame 2 on the direct strand.
RRRVRSRGPGVRTLMSQALMRPAQPAAQTGSSQIPKRNQSASERRAQPRRCWATSFAVSV
GTRPPASTTTCSAAKAARASSGAVWSVVGPGAMPAGVAEPARWTLSCGASASSAGCASAR
RQG*
>ORF number 3 in reading frame 2 on the direct strand extends from base 464 to base 661.
GGGAGCAGTGCGTCCTTTCTGAAGAACAGATCCGGAAGAAGAAGATTCGGAAACAACAGC
AGCAGGAGTCACAGTCACAGTCGCAGTCACCTGTGGGGCCGCAGGGCAGCAGCAGCTCAG
CCTCTGGGCCTGGGGCTTCCCCTGGTGGATCTGAGGCAGGCAGCCAGGGCTCCGGGGAAG
GCGAGGGTGTCCAGCTAA
>Translation of ORF number 3 in reading frame 2 on the direct strand.
GSSASFLKNRSGRRRFGNNSSRSHSHSRSHLWGRRAAAAQPLGLGLPLVDLRQAARAPGK
ARVSS*
>ORF number 4 in reading frame 2 on the direct strand extends from base 683 to base 913.
TCCAGCAGTTGGTGGCGGCCCAACTGCAGTGCAACAAACGCTCCTTCTCCGACCAGCCCA
AAGTCACGCCCTGGCCCCTGGGCGCAGACCCCCAGTCCCGAGATGCCCGCCAGCAACGCT
TTGCCCACTTCACGGAGCTGGCCATCATCTCAGTCCAGGAGATCGTGGACTTCGCTAAGC
AAGTGCCTGGTTTCCTGCAGCTGGGCCGGGAGGACCAGATCGCCCTCCTGA
>Translation of ORF number 4 in reading frame 2 on the direct strand.
SSSWWRPNCSATNAPSPTSPKSRPGPWAQTPSPEMPASNALPTSRSWPSSQSRRSWTSLS
KCLVSCSWAGRTRSPS*
Results for 1386 residue sequence "Untitled" starting "ATGTCCTCTC"
>ORF number 1 in reading frame 1 on the direct strand extends from base 1 to base 1386. ATGTCCTCTCCTACCACGAGTTCCCTGGATACCCCCCTGCCTGGAAATGGCCCCCCTCAG CCTGGCGCCCCTTCTTCTTCACCCACTGTAAAGGAGGAGGGTCCGGAGCCGTGGCCCGGG GGTCCGGACCCTGATGTCCCAGGCACTGATGAGGCCAGCTCAGCCTGCAGCACAGACTGG GTCATCCCAGATCCCGAAGAGGAACCAGAGCGCAAGCGAAAGAAGGGCCCAGCCCCGAAG ATGCTGGGCCACGAGCTTTGCCGTGTCTGTGGGGACAAGGCCTCCGGCTTCCACTACAAC GTGCTCAGCTGCGAAGGCTGCAAGGGCTTCTTCCGGCGCAGTGTGGTCCGTGGTGGGGCC AGGCGCTATGCCTGCCGGGGTGGCGGAACCTGCCAGATGGACGCTTTCATGCGGCGCAAG TGCCAGCAGTGCCGGCTGCGCAAGTGCAAGGAGGCAGGGATGAGGGAGCAGTGCGTCCTT TCTGAAGAACAGATCCGGAAGAAGAAGATTCGGAAACAACAGCAGCAGGAGTCACAGTCA CAGTCGCAGTCACCTGTGGGGCCGCAGGGCAGCAGCAGCTCAGCCTCTGGGCCTGGGGCT TCCCCTGGTGGATCTGAGGCAGGCAGCCAGGGCTCCGGGGAAGGCGAGGGTGTCCAGCTA ACAGCGGCTCAAGAACTAATGATCCAGCAGTTGGTGGCGGCCCAACTGCAGTGCAACAAA CGCTCCTTCTCCGACCAGCCCAAAGTCACGCCCTGGCCCCTGGGCGCAGACCCCCAGTCC CGAGATGCCCGCCAGCAACGCTTTGCCCACTTCACGGAGCTGGCCATCATCTCAGTCCAG GAGATCGTGGACTTCGCTAAGCAAGTGCCTGGTTTCCTGCAGCTGGGCCGGGAGGACCAG ATCGCCCTCCTGAAGGCATCCACTATCGAGATCATGCTGCTAGAGACAGCCAGGCGCTAC AACCACGAGACAGAGTGTATCACCTTCTTGAAGGACTTCACCTACAGCAAGGACGACTTC CACCGTGCAGGCCTGCAGGTGGAGTTCATCAACCCCATCTTCGAGTTCTCGCGGGCCATG CGGCGGCTGGGCCTGGACGACGCTGAGTACGCCCTGCTCATCGCCATCAACATCTTCTCG GCCGACCGGCCCAACGTGCAGGAGCCGGGCCGCGTGGAGGCGTTGCAGCAGCCCTACGTG GAGGCGCTGCTGTCCTACACGCGCATCAAGAGGCCGCAGGACCAGCTGCGCTTCCCGCGC ATGCTCATGAAGCTGGTGAGCCTGCGCACGCTGAGCTCTGTGCACTCGGAGCAGGTCTTC GCCTTGCGGCTCCAGGACAAGAAGCTGCCGCCTCTGCTGTCGGAGATCTGGGACGTCCAC GAGTGA
>Translation of ORF number 1 in reading frame 1 on the direct strand.
MSSPTTSSLDTPLPGNGPPQPGAPSSSPTVKEEGPEPWPGGPDPDVPGTDEASSACSTDW
VIPDPEEEPERKRKKGPAPKMLGHELCRVCGDKASGFHYNVLSCEGCKGFFRRSVVRGGA
RRYACRGGGTCQMDAFMRRKCQQCRLRKCKEAGMREQCVLSEEQIRKKKIRKQQQQESQS
QSQSPVGPQGSSSSASGPGASPGGSEAGSQGSGEGEGVQLTAAQELMIQQLVAAQLQCNK
RSFSDQPKVTPWPLGADPQSRDARQQRFAHFTELAIISVQEIVDFAKQVPGFLQLGREDQ
IALLKASTIEIMLLETARRYNHETECITFLKDFTYSKDDFHRAGLQVEFINPIFEFSRAM
RRLGLDDAEYALLIAINIFSADRPNVQEPGRVEALQQPYVEALLSYTRIKRPQDQLRFPR
MLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE*
>ORF number 1 in reading frame 2 on the direct strand extends from base 2 to base 91.
TGTCCTCTCCTACCACGAGTTCCCTGGATACCCCCCTGCCTGGAAATGGCCCCCCTCAGC
CTGGCGCCCCTTCTTCTTCACCCACTGTAA
>Translation of ORF number 1 in reading frame 2 on the direct strand.
CPLLPRVPWIPPCLEMAPLSLAPLLLHPL*
>ORF number 2 in reading frame 2 on the direct strand extends from base 92 to base 463.
AGGAGGAGGGTCCGGAGCCGTGGCCCGGGGGTCCGGACCCTGATGTCCCAGGCACTGATG
AGGCCAGCTCAGCCTGCAGCACAGACTGGGTCATCCCAGATCCCGAAGAGGAACCAGAGC
GCAAGCGAAAGAAGGGCCCAGCCCCGAAGATGCTGGGCCACGAGCTTTGCCGTGTCTGTG
GGGACAAGGCCTCCGGCTTCCACTACAACGTGCTCAGCTGCGAAGGCTGCAAGGGCTTCT
TCCGGCGCAGTGTGGTCCGTGGTGGGGCCAGGCGCTATGCCTGCCGGGGTGGCGGAACCT
GCCAGATGGACGCTTTCATGCGGCGCAAGTGCCAGCAGTGCCGGCTGCGCAAGTGCAAGG
AGGCAGGGATGA
>Translation of ORF number 2 in reading frame 2 on the direct strand.
RRRVRSRGPGVRTLMSQALMRPAQPAAQTGSSQIPKRNQSASERRAQPRRCWATSFAVSV
GTRPPASTTTCSAAKAARASSGAVWSVVGPGAMPAGVAEPARWTLSCGASASSAGCASAR
RQG*
>ORF number 3 in reading frame 2 on the direct strand extends from base 464 to base 661.
GGGAGCAGTGCGTCCTTTCTGAAGAACAGATCCGGAAGAAGAAGATTCGGAAACAACAGC
AGCAGGAGTCACAGTCACAGTCGCAGTCACCTGTGGGGCCGCAGGGCAGCAGCAGCTCAG
CCTCTGGGCCTGGGGCTTCCCCTGGTGGATCTGAGGCAGGCAGCCAGGGCTCCGGGGAAG
GCGAGGGTGTCCAGCTAA
>Translation of ORF number 3 in reading frame 2 on the direct strand.
GSSASFLKNRSGRRRFGNNSSRSHSHSRSHLWGRRAAAAQPLGLGLPLVDLRQAARAPGK
ARVSS*
>ORF number 4 in reading frame 2 on the direct strand extends from base 683 to base 913.
TCCAGCAGTTGGTGGCGGCCCAACTGCAGTGCAACAAACGCTCCTTCTCCGACCAGCCCA
AAGTCACGCCCTGGCCCCTGGGCGCAGACCCCCAGTCCCGAGATGCCCGCCAGCAACGCT
TTGCCCACTTCACGGAGCTGGCCATCATCTCAGTCCAGGAGATCGTGGACTTCGCTAAGC
AAGTGCCTGGTTTCCTGCAGCTGGGCCGGGAGGACCAGATCGCCCTCCTGA
>Translation of ORF number 4 in reading frame 2 on the direct strand.
SSSWWRPNCSATNAPSPTSPKSRPGPWAQTPSPEMPASNALPTSRSWPSSQSRRSWTSLS
KCLVSCSWAGRTRSPS*
